Fig. 1. PpMACRO2 sequence relationship and gene annotation.

a Multiple alignment for PpMACRO2 protein sequence and homologs sampled from different major groups. Boxes in the alignment show amino acids uniquely shared by bryophytes and Mucoromycota fungi. Detailed molecular phylogeny of PpMACRO2 and homologs is shown in Supplementary Figs. 4 and 5. b Schematic diagram of PpMACRO2 gene structure and encoded protein sequence based on RT-PCR and sequencing evidence. Exons 1, 2, and 3 correspond to exons 1, 3, and 4, respectively, of the P. patens v3.3 annotation in Phytozome.