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. 2020 Apr 24;11:2030. doi: 10.1038/s41467-020-15967-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a, b Protonemal cell growth and elongation in WT, ko and OE plants. Protonemal cell length in ko mutants decreased by about 40% than in the wild type after 19 days, but there was no significant difference between OE lines and the wild type. After 30 days, protonemal cells in ko mutants were only about 15% shorter compared to the wild type, whereas protonemal cells of OE lines were about 30% shorter. These results show that protonemata grow much faster in ko mutants but slower in OE lines compared to the wild type between day 19 and day 30. Micrograph images shown were observed from at least three biological replicates. Data show means ± s.e.m. of ten biological replicates for WT and mutant plants. c, d Sizes of gametophores developed from four-week-old WT, ko, and OE plants. Gametophore heights were measured twice independently, with similar results. Micrograph images provided were observed from five biological replicates. Data show means ± s.e.m. of five biological replicates. Scale bar: 200 μm in a; 500 μm in c.