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. 2020 Apr 24;11:2030. doi: 10.1038/s41467-020-15967-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Protoplast regeneration, with red arrowheads indicating differentiated cells and buds. b Tissue regeneration using detached leaves from WT plants, as well as ko and OE mutants of P. patens. Black arrows indicate emerged protonema filaments. c GUS staining and GFP fluorescence of leaves were observed from PpMACRO2-EGFP-GUS transgenic lines after being detached for 48 h. Micrograph images shown were observed from five biological replicates. Scale bars: 100 μm in a, c; 500 μm in b.