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. 2019 May 9;12(3):176–189. doi: 10.1093/jmcb/mjz040

Figure 3.

Figure 3

Knockdown of TRIM14 attenuated the expression of adhesion molecules and cytokines and monocyte adherence to HUVEC. (A) HUVECs were transfected with TRIM14 siRNAs or control siRNAs. Twenty-four hours later, the transfected cells were treated with 10 ng/ml TNF-α for 0, 4, 8, and 24 h. Expression of VCAM-1, ICAM-1, E-selectin, and VE-cadherin were determined by western blot analysis. (B) Relative fold changes of proteins after 8 h treatment of TNF-α were determined by densitometry and normalized to β-actin. Data are presented as mean ± SD (n = 3); *P < 0.01 by Student’s t-test. (C) TRIM14 siRNAs or control siRNAs were transiently transfected into HUVECs. Transfected cells were quiescent for 24 h and then treated with 10 ng/ml TNF-α or PBS (as controls) for 4 h. The mRNA levels of adhesive molecules and cytokines were determined by qPCR and normalized to β-actin. Data are presented as mean ± SD (n = 3); *P < 0.05, **P < 0.01 by Student’s t-test. (D) TRIM14 siRNAs or control siRNAs were transfected into HUVECs, and the transfected cells were incubated with TNF-α or PBS for 8 h and then co-cultured with fluorescence-labeled THP-1 cells for 1 h. After carefully washing, adhesive cells were visualized and counted as described as above. ***P < 0.001.