Figure 7.

α-tubulin deacetylation and microtubule destabilization in photoreceptors of Hsp90α-deficient mice. (A) α-tubulin deacetylation in photoreceptors of Hsp90α-deficient mice. IFT88 was stained on retinal sections to show the CC. Scale bar, 10 μm. (B and C) Analysis of α-tubulin deacetylation by western blot. The retinas of wild-type (+/+), heterozygous (−/+), and homozygous (−/−) Hsp90α-deficient mice at the age of 3 or 6 weeks were isolated for western blot. (D) Quantification of acetylated α-tubulin. Acetylated α-tubulin on western blot was subjected to densitometry analysis and normalized by total α-tubulin. Fold expression of acetylated α-tubulin was calculated for Hsp90α deficiency relative to wild-type and heterozygote. Data are mean ± SEM of the results obtained from at least two mice for each group. *P < 0.05 (Student’s t-test).