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TRIM32 mediates non-proteolytic ubiquitination of OTULIN. (A) HEK293 cells were transfected with different doses of TRIM32. After 48 h, cell lysates were blotted with the indicated antibodies. (B) OTULIN-FLAG was transfected with wild-type ubiquitin or the indicated mutant into HEK293 cells. After 48 h, cell lysates were immunoprecipitated with anti-FLAG antibody and blotted as indicated.
