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. 2019 Sep 3;12(3):202–215. doi: 10.1093/jmcb/mjz090

Figure 2.

Figure 2

Factors secreted by OTCs activate PI3K/Akt, MAPK, and NF-κB signaling pathways and modify gene expression in MSCs. (A) Akt, phospho-Akt (Ser473), Erk, and Phospho-Erk expression levels were assayed by western blot on physiological BM-MSC or MSC/igrov-1 (representative of three experiments). (B) BM-MSCs were cultured with IGROV-1 CM or with control medium (control) for the indicated amount of time. T represents treatment of the BM-MSCs with 50 ng/ml of TNFα. NF-κB and phospho-NF-κB p65 protein expression was assayed by western blot. (C) Representative gene expression of physiological BM-MSCs (n = 6) and iCA-MSCs (MSC/igrov-1) (n = 6). RNA was extracted and analyzed by Nanostring™ technology. (D) Quantification of the transcriptomic analysis showing the log2 fold change of the selected genes with an initial filter (SD > 0.25) to eliminate genes with little variation. An ANOVA test was applied to compare the six BM-MSC control samples versus the six iCA-MSC (MSC/igrov-1) samples. The P-values were corrected for multiple tests using the BH method.