Skip to main content
. 2020 Apr 24;10:6993. doi: 10.1038/s41598-020-63726-w

Figure 7.

Figure 7

In vivo (first and third column) and in vitro (second and fourth column) effects of FGF21 on the mRNA expression of genes involved in lipid metabolism, mitochondrial activity and transcription factors in zebrafish. (a,c,e,g,i,k,m,o,q,s,u,w) Gene expression in the zebrafish liver 1 h after intraperitoneal administration of saline alone (control) or containing 1, 10 or 100 ng/g bw of FGF21. Data are expressed as mean + SEM (n = 6). Asterisks denote significant differences between control and treated groups assessed by t-test (*p < 0.05, **p < 0.01, ***p < 0.001). (b,d,f,h,j,l,n,p,r,t,v,x) Levels of mRNAs in ZFL cells exposed to 0.1, 1 and 10 nM FGF21 during 1 and 6 h. Data are shown as mean + SEM of the results obtained in two different experiments (n = 6 in each experiment). Asterisks denote significant differences between control and treated groups (*p < 0.05, **p < 0.01, ***p < 0.001). acaca, acetyl-CoA carboxylase; acadm, acyl-CoA dehydrogenase medium-chain; acat1, acetyl-CoA acetyltransferase 1; acly, ATP citrate lyase; atp5f1b, ATP synthase F1 subunit beta; cpt1a, carnitine palmitoyltransferase 1a; echs1, enoyl-CoA hydratase short-chain 1; fasn, fatty acid synthase; FGF21, fibroblast growth factor 21; hmgcl, 3-hydroxy-3-methylglutaryl-CoA lyase; hadh, 3-hydroxyacyl CoA dehydrogenase; pparα, peroxisome proliferator-activated receptor alpha; ppargc1α, peroxisome proliferator-activated receptor gamma coactivator 1-alpha.