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. 2020 Apr 23;10:6876. doi: 10.1038/s41598-020-63787-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of alpha diversity and relative abundance at phylum level based on the OTUs profile. Box plots depict differences in microbiome diversity based on (A) Shannon index and (B) ACE index between patients with dysspermatism and healthy controls. (C,D) show Shannon index and ACE index among patients with asthenospermia, oligospermia, oligoasthenospermia, and azoospermia, and healthy controls. The p value was calculated using the Wilcoxon rank-sum test.