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. 2020 Apr 23;11(4):266. doi: 10.1038/s41419-020-2448-6

Fig. 1. Levels of the self-renewal protein Nanog is increased in HNSCC spheroid cells, CD44(+) cells, and patient tumor tissues.

Fig. 1

a, b Graphs of the positive area (%) per field for the CSC marker proteins CD24, CD44, CD133, and Musashi-1 and the self-renewal proteins Sox2, Oct-4, Nanog, and c-Myc. c Immunofluorescence (IF) staining of commercially available tissue array slides containing 45 HNSCC and 5 corresponding normal tissues with DAPI (blue), CD44 (green), and Nanog (red). Scale bar = 20 µm. d, e Western blotting of CSC marker proteins and self-renewal proteins in three HNSCC cell lines grown as monolayers or as spheroids. f Western blotting of CD44, Sox2, Oct-4, Nanog, and c-Myc following FACS for CD44(−) and CD44(+) cells. g IF of CD44(+) SCC-15 and QLL-1 cells transduced with sh.NANOG or sh.Scr. Scale bar = 50 µm. h GFP-expressing cell in a single cell assay using CD44(+) SCC-15 and QLL-1 cells following transduction with GFP, or NANOG shRNA (sh.Nanog), or scrambled shRNA (sh.Scr). Error bars represent standard deviation. *p < 0.05.