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. 2020 Apr 23;11(4):266. doi: 10.1038/s41419-020-2448-6

Fig. 3. Nanog inhibition in CD44(+) HNSCC cells relieves radiotherapy resistance.

Fig. 3

a Western blotting of Nanog in CD44(+) cells transduced with sh.NANOG or sh.Scr. b Proliferation assays of CD44(+) HNSCC cells following treatment with sh.NANOG, sh.Scr, or 8 Gy radiotherapy. c Tumor growth curves of CD44(+) SCC-25 cell xenografts after treatment with sh.NANOG, sh.Scr, or 8 Gy. d Representative tumor images from each treatment group on the 15th day. e Immunofluorescence (IF) analysis of treated tumors for proliferation (Ki-67, green), apoptosis (cleaved caspase-3, red), ɣH2AX (DNA damage, white), and CD44 (green). Scale bar = 20 µm. Error bars represent standard deviation. *p < 0.05.