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. 2020 Apr 23;3:188. doi: 10.1038/s42003-020-0922-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Representative confocal images showing mRNA expression of CTHRC1 (green) and APCDD1 (red), selected markers for the secretory-reticular and secretory-papillary fibroblast subpopulations, respectively. Details from the papillary and reticular regions of the images above are shown in the lower panels (left and center, respectively), and percentage of positive cells for each gene and per dermal region are shown in the lower right panel. b Representative confocal images showing mRNA expression of CCL19 (green) and APOE (red), selected markers for the pro-inflammatory fibroblast subpopulation. A detail of a vessel of the images above is shown in the lower panel. c Representative confocal images showing mRNA expression of ASPN (green), selected marker for the mesenchymal fibroblast subpopulation. A detail of the hair follicle bulb of the images above is shown in the lower panel. Dashed lines in a and b denote the papillary dermis regions while in c denote the dermal papilla. Nuclei were counterstained with DAPI. Each assay was performed in three independent young FFPE skin sections (28–37 y/o). Images are shown at ×40 original magnification. Scale bar: 50 μm for main images and 10 μm for detail images. Pap papillary dermis, Ret reticular dermis, Deep ret deep reticular dermis, HF hair follicle, DP dermal papilla. Statistical analyses were performed using a two-way ANOVA test (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001); error bars represent the standard deviation.