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. 2020 Apr 23;11:1943. doi: 10.1038/s41467-020-15732-9

Fig. 1. TGF-β1 leads to a decrease of SNCA expression in HK-2 in vitro.

Fig. 1

Human renal proximal tubular epithelial (HK-2) cells were incubated in serum-free medium (control) or TGF-β1 for 24 h (a), 48 h (a) and 72 hours (a, b–f). a Total mRNA was extracted from HK-2 cells and mRNA levels of SNCA were determined by quantitative real-time PCR and normalized to GAPDH. Data are presented as mean ± SEM of at least n = 3 independent experiments. b Cell lysates were immunoblotted with antibodies against E-cadherin, SNCA, vimentin and α-SMA. The same samples were reprobed with antibodies against tubulin to ensure equal loading. Representative Western blots (b) and quantitative densitometric analysis (c–f) show decrease of E-cadherin and SNCA expression and an increase of vimentin and α-SMA expression in HK-2 cells treated with TGF-β1 for 72 h. Data are presented as mean ± SEM of at least n = 2 independent experiments. g Immunofluorescence staining for the distribution of SNCA in HK-2 cells after incubation with TGF-β1 for 72 h. Scale bar represents 20 µm. *p < 0.05, **p < 0.01, ***p < 0.001. The p-value by one-way ANOVA. Source data are provided as a Source Data file.