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. 2020 Apr 24;11(4):282. doi: 10.1038/s41419-020-2483-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a HUVEC were treated with TNFα (50 ng/ml) for the indicated times, then MLKL content in the cytoplasmic and nuclear fraction was determined by immunoblot. b–d HUVEC were lentivirally transduced with MLKL-HA and MLKL-HA-NLS followed by TNFα (50 ng/ml) treatment for 6 h, before the expression and localization of MLKL-HA or MLKL-HA-NLS in HUVEC was confirmed by immunofluorescent staining against HA tag (b). The expression of ICAM1, VCAM1, and E-selectin was determined by real-time PCR (c) and immunoblot (d). Data are representative of three independent experiments, with each experiment containing biological triplicates. Mean ± SD; **P < 0.01, ***P < 0.001; ns, not significant; Student’s t test.