Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Apr 24;10:6919. doi: 10.1038/s41598-020-62955-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Design and manufacture of Cardiac MicroRing (CaMiRi) platform. (A) Process flow of substrate manufacturing. The process started with creating a three-dimensional Computer Aided Design (3D CAD) model of the plate. The design is then printed using an Objet 30 Pro 3D printer to generate a positive mold. A negative PDMS mold is then created which is followed by a complementary positive PDMS mold. This positive PDMS mold is used to then create a polyurethane-based negative master mold which can be used to mold multiple PDMS plates. (B) 3D CAD model of a plate for 3D printing (top panel) and actual 3D-printed mold (bottom panel). Scale bars represent 20 mm. (C) Cross section of a well showing the seeding reservoir where cells are pipetted and the microcantilevers used for force-displacement measurement (empirically calibrated with a force transducer (Micro-Squisher) with tissue anchor points to hold microtissue in place. Scale bars represent 1 mm. (D) Tissue anchor angles and lengths were tested to determine optimal anchor geometry. Scale bars represent 1 mm. (E) Force-displacement curve of cantilevers as measured with the Micro-Squisher. (F) Cell-laden collagen seeded into reservoirs of CaMiRi device remodel into cardiac tissues tethered around microcantilevers within 24 hours. 9 wells of a 96-well plate containing CaMiRi are also shown. Scale bars represent 5 mm. (G) CaMiRi formulated from a range of total input cells (25,000; 50,000; 100,000; and 150,000 cells seeded per well. Scale bars represent 1 mm. (H) Live/dead staining of CaMiRi. From left to right: bright field, nuclei in blue, live cells in green, and dead cells in red. Scale bars represent 1 mm.