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. 2020 Apr 24;10:6919. doi: 10.1038/s41598-020-62955-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CaMiRi response to cardiotropic drugs and growth factors. Drugs were administered and tissues incubated for 20 minutes before contractile force assay. Along with the vehicle control, up to five drug concentrations of 1 nm, 10 nm, 100 nm, 1 µm, and 10 µm are administered. Data shown are relative change in contraction amplitude due to the administration of (A) Blebbistatin (negative inotrope), (B) Isoproterenol (positive inotrope), and (C) Nifedipine (negative inotrope), as compared to the vehicle control. Data are reported from three independent experiments, each with three replicates, as the mean ± standard deviation. *P < 0.05.