Table 2.
Mean square values of the analysis of variance of oxidized glutathione (GSSG) (nmole g−1 FW), total glutathione (GT) (nmole g−1 FW), reduced glutathione (GSH) (nmole g−1 FW), GSH/GSSG, Glutathione reductase (GR) (µmol min−1 mg protein−1), catalase (CAT) activity (U mg−1 protein), peroxidase (POD) activity (U mg−1 protein) and superoxide dismutase (SOD) and activity (U mg−1 protein).
Source of variants | df | GSSG | GT | GSH | GSH/GSSG | GR | CAT | POD | SOD |
---|---|---|---|---|---|---|---|---|---|
Species (S) | 1 | 85.71** | 30731.13** | 36352.05** | 31.75ns | 0.0004** | 0.0004** | 0.004** | 0.007** |
Drought (D) | 2 | 20875.64** | 521364.43** | 188251.46** | 33110.52** | 140.29** | 0.02** | 0.63** | 0.004** |
Melatonin (M) | 4 | 281.50** | 40178.05** | 53509.01** | 1092.27** | 3.49** | 0.0008** | 0.01** | 0.001** |
S × D | 2 | 63.56** | 2517.30** | 3992.04** | 15.44ns | 0.47** | 0.000003 ns | 0.0004* | 0.003** |
S × M | 4 | 8.28** | 1072.17** | 806.11** | 6.62ns | 0.01 ns | 0.00006 ns | 0.00008 ns | 0.0002** |
D × M | 8 | 80.54** | 6338.33** | 9166.92** | 345.67** | 0.08** | 0.00008* | 0.003** | 0.0001** |
S × D × M | 8 | 3.88** | 418.35* | 346.75* | 4.54ns | 0.05* | 0.00005ns | 0.00006ns | 0.0003** |
Error | 60 | 0.6 | 158.12 | 190.19 | 11.76 | 0.02 | 0.00003 | 0.00008 | 0.000002 |
CV (%) | 2.98 | 3.80 | 4.95 | 11.62 | 3.42 | 10.87 | 6.23 | 4.74 |
* and **: Significant at the 5% and 1% probability levels, respectively., ns: Non- Significant, according to the LSD multiple range test at P ≤ 0.05. df, degrees of freedom.