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. 2020 Apr 24;3:192. doi: 10.1038/s42003-020-0924-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Bacterial two-hybrid analysis of the interaction between Glk and CobB or Kct1. Glk was constructed in pT18, while CobB or Kct1 in pT25. E. coli strain BTH101 containing the hybrids were cultured on LB for 48 h and photographed. b, c In vitro assays for Glk crotonylation by Kct1 (b) and decrotonylation by CobB (c). Reactions were analyzed by western blot with the indicated antibodies (1:2000). The relative intensity of each sample is also shown. In b, the reaction time is 1 h, while in c, the reaction time for decrotonylation is: lane 1, 1.5 h; lane 2, 1 h; lane 3, 1.5 h.