Fig. 1. DSB-induced R-loops form in a transcription-dependent manner.

Boxplots (top) and metagenes (bottom) of R-loop generation at ASISI-induced double-strand breaks. a The 99 breaks are grouped by their repair pathway preference (determined by Aymard et al.⁴¹). b Breaks are grouped by their genomic location status. c Breaks are grouped by their relative transcriptional activity (determined by Aymard et al.⁴¹). DRIP read coverage was calculated and normalised to the total read number; then the log2 fold change of broken (+4OHT) over unbroken (−4OHT) was calculated. Statistics were calculated using a directional, unpaired Wilcoxon signed-rank non-parametric test, *p < 0.05, **p < 0.01.