Fig. 4.

Reduced expression of BTBD9 leads to TNFAIP1 accumulation, promoting metastasis in lung cancer. a The effects of BTBD9 and TNFAIP1 knockdown in A549 cells were confirmed using western blotting. b The effects of BTBD9 and TNFAIP1 knockdown in H1299 cells were confirmed using western blotting. c, d The Transwell assay was used to detect the migration ability of A549 and H1299 cells in which TNFAIP1 and BTBD9 were downregulated individually or together (scale bar = 100 μm). Data are presented as the mean ± SD (n = 5), p < 0.001 vs the sgRNA-BTBD9 group. These data are representative of three independent experiments, the results of which showed similar trends