Fig. 1. ApoJ levels in serum and multiple organs of L-ApoJ^−/− mice.

a ApoJ protein levels in serum of liver-specific ApoJ-deficient mice (L-ApoJ^−/−). Serum was separated by SDS–PAGE. ApoJ or ApoB 48 was visualized by immunoblotting. These data are representative from more than three independent experiments. b ApoJ protein levels in multiple metabolic organs of L-ApoJ^−/−. Tissue lysates (20–50 μg) were separated by SDS–PAGE. ApoJ or actin was visualized by immunoblotting. These data are representative from more than three independent experiments. Mus muscle, Adi adipose tissue, Hypo hypothalamus, Panc pancreas, Kid kidney, Stom stomach, Intes intestine, Spl spleen, BAT brown adipose tissue; C: ApoJ^loxP/loxP mice, KO: albumin-Cre: ApoJ^loxP/loxP mice (L-ApoJ^−/−). c mRNA levels of ApoJ in multiple metabolic organs of L-ApoJ^−/− mice. mRNA levels of ApoJ were measured by RT-PCR. Mice were studied at 12 weeks of age. Molecular marker (MM) is 100 bp DNA ladder. 313 bp indicates ApoJ mRNA. These data are representative from three independent experiments; Con: ApoJ^loxP/loxP mice, KO: albumin-Cre: ApoJ^loxP/loxP mice (L-ApoJ^−/−).