Fig. 2. Liver is a major source of circulating ApoJ.

a ApoJ protein levels in serum of L-ApoJ^−/− mice injected with ApoJ or GFP adenovirus. L-ApoJ^−/− or global ApoJ^−/− mice were injected with a recombinant adenovirus encoding a secretory ApoJ or GFP at a concentration of 2 × 0⁹ pfu per gram of body weight via the tail vein. A: ApoJ-expressing adenovirus, G: GFP-expressing adenovirus. Serum was separated by SDS–PAGE. ApoJ, ApoB 48 or actin were visualized by immunoblotting. b ApoJ protein levels in multiple metabolic organs of in L-ApoJ^−/− mice injected with ApoJ or GFP adenovirus. Tissue lysates (20–50 μg) were separated by SDS–PAGE. ApoJ or GAPDH were visualized by immunoblotting. c ApoJ protein levels in serum of in global ApoJ^−/− mice injected with ApoJ or GFP adenovirus. d ApoJ protein levels in multiple metabolic organs of global ApoJ^−/− mice. Tissue lysates (20–50 μg) were separated by SDS–PAGE. All data are representative from three independent experiments. Mice were studied at 9–10 weeks of age. Hypo hypothalamus, BAT brown adipose tissue.