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. 2020 Apr 17;11:495. doi: 10.3389/fphar.2020.00495

Figure 2.

Figure 2

Downregulation of c-Myc enhanced the inhibition effect of bufalin on cell proliferation and cell cycle in pancreatic cancer cells. PANC-1 and SW1990 cells were transfected with si-c-Myc and pcDNA-c-Myc, respectively. Cells were treated with dimethyl sulfoxide (DMSO) or bufalin (80 nmol/L) for 24 h. (A) The structure of bufalin. (B) The viability of PANC-1 and SW1990 cells were detected via CCK-8 assay (* p < 0.05, ** p < 0.01 vs control, n = 3). (C) Cell cycle distribution was analyzed via flow cytometry. (D) Statistical histograms of cells in the G1/G0, S, and G2/M phases of the cell cycle (* p < 0.05, ** p < 0.01 vs control, ▲▲ p < 0.01 vs bufalin treatment group, n = 3).