Figure 5.

Growth of transgenic lines after ABA treatment. (A) Root phenotype and (B) root length of WT and transgenic lines grown in the presence or absence of 10 µM ABA. (C) Number of lateral roots and (D) lateral root density in plants grown on medium supplemented with ABA. The graphs represent three biological replicates with minimum 17 data points per condition. The seeds of WT plants and transgenic lines (KO1, KO2, OE3, and OE4) were germinated in vitro for 3 days and the seedlings were transferred onto a medium containing 10 µM ABA for another 7 days. The comparison between the root length of WT and transgenic lines was made by using Independent-samples T-test for normally distributed data. The statistical analysis for the number of lateral roots and lateral root density was performed using Mann-Whitney U test for not normally distributed data. The distributions of root length, number of lateral roots, and lateral root density values are shown as box and whisker plots. The boxes represent the 25–75th percentiles, the median is indicated as a horizontal line, the mean is indicated as a cross, the outliers are represented as circles. The whiskers are equal to 1.5× the interquartile range (***p < 0.001).