REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Fluorescein labeled Lotus Tetragonolobus (LTL)	Vector Labs	Cat#FL-1321; RRID:AB_2336559
Anti-SLC3A1 polyclonal antibody	Merck	Cat#HPA038360-100U; RRID:AB_2675975
Anti-SGLT2	Abcam	Cat#ab37296; RRID:AB_777895
Anti-LAMININ	Merck	Cat#L9393; RRID:AB_477163
Human Nephrin Affinity Purified Polyclonal Ab antibody	R&D Systems	Cat#AF4269; RRID:AB_2154851
Recombinant Anti-Sodium Potassium ATPase antibody	Abcam	Cat#ab209299; RRID:AB_1968790
Bacterial and Virus Strains
SARS-CoV-2, GENBANK: MT093571	Isolated from patient	N/A
Chemicals, Peptides, and Recombinant Proteins
CHIR99021	Merck	Cat#SML1046; CAS: 252917-06-9
Recombinant human FGF9	PeproTech	Cat#100-23
Heparin	Merck	Cat#H3149; CAS: 9041-08-1
Activin A	Vitro	Cat#338-AC-050
Paraformaldehyde solution 4% in PBS	Santa Cruz	Cat#sc-281692
1% Triton X-100	Merck	Cat#T8787
Glutaraldehyde	Sigma-Aldrich	Cat#G7776
srhACE2	Apeiron	N/A
Trizol	ThermoFisher	Cat#15596018
Recombinant Human VEGF165	Peprotech	Cat#100-20
Human FGF-2	Miltenyi Biotech	Cat#130-093-841
Critical Commercial Assays
streptavidin/biotin blocking kit	Vector Labs	Cat#SP-2002
CellTiter-Glo® Luminescent cell viability assay	Promega	Cat#G7570
Direct-zol RNA MiniPrep kit	Zymo Reasearch	Cat#R2051
Chromium Single Cell 3′ Library & Gel Bead Kit V3	10X Genomics (USA)	Cat#PN-1000075
NSQ 500/550 Hi Output KT v2.5 (75 CYS)	Illumina (San Diego, CA 92122 USA)	Cat#20024906
Sytox® blue dead cell stain	Thermofisher (Eugene, Oregon,USA)	Cat#S34857
Deposited Data
Kidney Organoid scRNA-seq	This paper	GEO: GSE 147863
Experimental Models: Cell Lines
ES[4] Human Embryonic Stem Cell line	The National Bank of Stem Cells (ISCIII,Madrid)	https://www.isciii.es/QueHacemos/Servicios/BIOBANCOS/BNLC/Lists/Lneas%20embrionarias/Attachments/6/Caracteristicas%20-%20Documento_Deposito_Lineas_v32_ES4_def.pdf
Vero E6 cells	ATCC	CRL-1586
Oligonucleotides
Primer: RPLP0	N/A	N/A
Forward: CCATTCTATCATCAACGGGTACAA
Reverse: AGCAAGTGGGAAGGTGTAATCC
Primer: SLC3A1	N/A	N/A
Forward: CACCAATGCAGTGGGACAAT
Reverse: CTGGGCTGAGTCTTTTGGAC
Primer: SLC27A2	N/A	N/A
Forward: TACTCTTGCCTTGCGGACTAA
Reverse: CCGAAGCAGTTCACCGATATAC
Primer: SLC5A12	N/A	N/A
Forward: ACACGGTACAGACCTTCGTCA
Reverse: GCTGCTCCCAGGTATTTGTC
Primer: SARS-CoV-2 E gene	N/A	N/A
Forward: ACAGGTACGTTAATAGTTAATAGCGT
Reverse: ATATTGCAGCAGTACGCACACA
Primer: Human RNase P	N/A	N/A
Forward: AGATTTGGACCTGCGAGCG
Reverse: GAGCGGCTGTCTCCACAAGT
Recombinant DNA
Human RNase P probe: FAM-TTCTGACCTGAAGGCTCT GCGCG-MGB	N/A	N/A
SARS-CoV-2 E gene probe: FAM-ACACTAGCCATCCTT ACTGCGCTTCG-QSY	N/A	N/A
Software and Algorithms
GraphPad Prism 8 (GraphPad)	Motulsky and Brown, 2006	https://www.graphpad.com
ImageJ	Schneider et al., 2012	https://imagej.net/ImageJ
FACSDiva software version 8.0.1 (BD Biosciences)	Becton, Dickinson and Company	https://www.bdbiosciences.com/en-us/instruments/research-instruments/research-software/flow-cytometry-acquisition/facsdiva-software
FlowJo software version 10	Becton, Dickinson and Company	https://www.flowjo.com/
Cell Ranger v3.0.1	10X Genomics	https://support.10xgenomics.com/single-cell-gene-expression/software/overview/welcome
R v3.5.1	R Core	https://cran.r-project.org/
Seurat v3.0.2	Stuart et al., 2019	https://satijalab.org/seurat/
Kidney Interactive Transcriptomics (KIT)	Wu et al., 2018a	http://humphreyslab.com/SingleCell/