Skip to main content
. Author manuscript; available in PMC: 2020 Aug 24.
Published in final edited form as: ACS Nano. 2019 Dec 2;13(12):13884–13898. doi: 10.1021/acsnano.9b05037

Figure 1. Development and characterization of iron oxide loaded RNA-nanoparticles.

Figure 1.

a, Representative Cryo-TEM of RNA-NPs with or without iron oxide (IO). b, Size distribution of RNA-NPs with and without IO (100ug IO:1mg lipid) assessed by Nanosight. c, Saturation magnetization of IO-RNA-NPs (100ug IO:1mg lipid) assessed with a SQUID magnetometer. d, Agarose gel electropheresis demonstrating RNA bound by different formulations of IO-RNA-NPs (labelled as the mass of IONPs in each formulation per mg lipid) after 15 minute incubation with RNA at different lipid:RNA ratios. e, RNA-binding capacity for RNA-NPs containing varying amounts of iron oxide. Numbers on graph are p values derived from one-way ANOVA and Tukey’s tests with n=4 per group. f, Viability of DC2.4s after 24 hour incubation with RNA-NPs assessed by flow cytometry (n=3). g, Uptake of Cy5-labelled RNA by DC2.4s assessed by flow cytometry after overnight incubation with RNA-NPs (n=3).