Figure 1.

The human FSHB transgene is activin-regulated in pituitary cultures. Pituitary cells were isolated from adult human FSHB transgenic female (A, B) and male (C, D) mice. Cells were treated for 6 h with 1 nM activin A or activin B. Relative murine Fshb (A and C, black bars) and human FSHB (B and D, aqua bars) mRNA levels were determined by RT-qPCR using species-specific primers and normalized by Rpl19 expression. (E and F) Pituitary cells were isolated from male hFSHB transgenic mice. Cells were treated with DMSO or SB431542 (10 µM) for 2, 6, 12, or 24 h. Murine Fshb (E) and human FSHB (F) mRNA expression were determined as above. (G and I) Pituitary cells were isolated from male hFSHB transgenic mice. Cells were treated with DMSO or actinomycin D (10 µg/mL) for 2 or 6 h. Murine Fshb (G) and human FSHB (I) mRNA expression were determined previously described. Data represent the mean (+standard error of the mean) of 3 independent experiments. *P < 0.05, **P < 0.01, and ***P < 0.005.