Figure 2.
FOXL2 regulates human FSHB expression in pituitary cultures. Pituitary cells were isolated from adult hFSHB/+;Foxl2fx/fx female (A, C, E) and male (B, D, F) mice. After in vitro recombination with a Cre-expressing adenovirus (Cre; a GFP-expressing virus was used as control), cells were treated with activin A (1 nM), activin B (1 nM), follistatin 288 (FST288, 200 ng/ml), or SB431542 (10 µM) for 6 h. RNA was collected and murine Fshb (A, B), human FSHB (C, D), Lhb (E, F), and Foxl2 (G, H) expression determined by RT-qPCR. Data represent the mean (+standard error of the mean) of 3 independent experiments/sex. *P < 0.05, **P < 0.01, ***P < 0.005, or ****P < 0.001; n.s., not statistically different vs. GFP treatment.