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. 2020 Apr 17;11:427. doi: 10.3389/fpls.2020.00427

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Copyright © 2020 Rana, Rajurkar, Kumar and Mohankumar.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of ingestion of transgenic tobacco plant tissues expressing dsSlCHSA on S. litura larval body weight, lethality, and expression level. (A) The growth of S. litura after feeding detached mature tobacco leaves expressing dsSlCHSA (E4, E5 E6, E9, and E11). (B) Feeding caused significantly higher lethality than in control. (C) SlCHSA mRNA level in S. litura at 24, 72, 120, and 168 h after feeding. Three biological replicates, each consist of pooled RNA from three to five larvae were used for analysis. The RT-qPCR data were analyzed using the delta–delta Ct method. Housekeeping gene, Actin used as internal control. The mRNA level in the treated group was relative to control group at the same time point. Error bars indicate standard error of mean. Statistical significance of difference was analyzed with ANOVA (P = 0.05). Means with different letters are significantly different (Tukey’s test).