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. 2020 Apr 24;15(4):e0232015. doi: 10.1371/journal.pone.0232015

Fig 2. Removal of magnesium ions and ribonuclease digestion are required to destabilize the ribosome particle.

Fig 2

A) Native RNA gel: Left- RNA size markers; Lane 1- RNA extracted from E. coli shows intact ribosome 50S and 30S subunits; Lane 2- Lysate containing 10 mM EDTA and SUPERase In. The bright band at the bottom of Lanes 1 and 2 is digested RNA. The increased intensity of digested RNA in Lane 2 versus Lane 1 is due to the loss of SUPERase In activity after the 2 hour long NMR experiment. Lane 3- Ribosome preparation containing 10 mM EDTA treated with RNase A for 1 h. B) Denaturing protein gel: Left- Protein MW markers; Lane 1- Whole cell lysate; Lane 2- Lysate precipitate following treatment with RNase A for 2 h; Lane 3- Purified ribosome preparation.