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. 2020 Apr 24;15(4):e0232093. doi: 10.1371/journal.pone.0232093

Fig 2. BoHV5 subtyping.

Fig 2

(a) Amplification of UL27 and UL54 genes of BoHV5 by multiplex PCR resulted in amplification of 534 bp and 649 bp fragments respectively. (b) Digestion of PCR products (multiplex-UL27 and UL54 genes) by BstEII resulted in amplification of 420 bp, 363 bp, 249 bp and 171 bp fragments.