Table 3. Differentially expressed gene selected for RT-qPCR validation.
| KEGG pathway name | Symbol | Gene Description | Log2FC | p-value |
|---|---|---|---|---|
| Axon Guidance | Cxcl12 | chemokine (C-X-C motif) ligand 12 | 1.10 | 2.39E-89 |
| ECM-receptor interaction | Col1a2 | collagen, type I, alpha 2 | 5.74 | 1.69E-15 |
| Thbs2 | thrombospondin 2 | 2.67 | 4.62E-12 | |
| Pathways in cancer | Arnt2 | aryl hydrocarbon receptor nuclear translocator 2 | 1.70 | 1.28E-111 |
| Fgf7 | fibroblast growth factor 7 | 1.28 | 6.02E-86 | |
| Mmp2 | matrix metallopeptidase 2 | 5.28 | 1.77E-13 | |
| Cell adhesion molecules | Cdh4 | cadherin 4 (retinal) | 3.23 | 2.45E-153 |
| Cd80 | CD80 antigen | -3.52 | 7.99E-27 | |
| Cldn1 | claudin 1 | 4.64 | 4.05E-173 | |
| Cdh3 | cadherin 3 (placental) | 3.75 | 3.80E-54 | |
| TGF-β signaling | Gdf5 | growth differentiation factor 5 | 4.65 | 2.08E-11 |
| Lefty1 | left right determination factor 1 | 2.62 | 1.32E-12 | |
| Bmp7 | bone morphogenetic protein 7 | -2.31 | 2.04E-59 | |
| Bmp8b | bone morphogenetic protein 8b | 8.85 | 0 | |
| Thbs2 | thrombospondin 2 | 2.67 | 4.62E-12 | |
| Focal adhesion | Col1a2 | collagen, type I, alpha 2 | 5.74 | 1.69E-15 |
| Thbs2 | thrombospondin 2 | 2.67 | 4.62E-12 | |
| Neuroactive ligand- receptor interaction | Cysltr1 | cysteinyl leukotriene receptor 1 | 8.34 | 1.93E-30 |
| Drd4 | dopamine receptor D4 | 3.32 | 1.86E-11 | |
| Metabolic pathways | Aldh1a7 | aldehyde dehydrogenase family 1, subfamily A7 | 3.34 | 1.25E-25 |
| Cbr2 | carbonyl reductase 2 | 2.20 | 3.12E-10 | |
| B3gnt3 | UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 3 | -5.21 | ||
| Cbr3 | carbonyl reductase 3 | -1.05 | 2.114E-16 | |
| Ldhb | lactate dehydrogenase B | 2.15 | 7.57E-21 | |
| Gatm | glycine amidinotransferase (L-arginine:glycine amidinotransferase) | -4.63 | 6.11E-40 | |
| Calcium signaling | Adcy7 | adenylate cyclase 7 | 1.34 | 2.44E-15 |
| Cacna1d | calcium channel, voltage-dependent, L type, alpha 1D subunit | 3.87 | 5.65E-13 | |
| Cysltr1 | cysteinyl leukotriene receptor 1 | 8.34 | 1.93E-30 | |
| Cytokine-cytokine receptor interaction | Ccl7 | chemokine (C-C motif) ligand 7 | -2.40 | 9.08E-10 |
| Bmp7 | bone morphogenetic protein 7 | -2.31 | 2.04E-59 | |
| Cxcl11 | chemokine (C-X-C motif) ligand 11 | 2.96 | 2.05E-20 | |
| Cxcl12 | chemokine (C-X-C motif) ligand 12 | 1.10 | 2.39E-89 | |
| Gdf5 | growth differentiation factor 5 | 4.65 | 2.08E-11 | |
| MAPK signaling | Mapk13 (P38-delta) | mitogen-activated protein kinase 13 | 1.66 | 5.24E-52 |
| Cacna1d | calcium channel, voltage-dependent, L type, alpha 1D subunit | 3.87 | 5.65E-13 | |
| Fgf7 | fibroblast growth factor 7 | 1.28 | 6.02E-86 |
The expression of selected genes related to the KEGG pathways were validated by RT-qPCR. LogFC and p-values were obtained from RNAseq analysis.