Table 4. Primers to validate differentially express genes.
Gene | Amplicon size (bp) | primer | Primer Sequence (5'->3') | Length | Gene accession |
---|---|---|---|---|---|
Cxcl12 | 116 | F | ctgtgcccttcagattgttg | 20 | NM_001012477.2 |
R | ctctgcgccccttgttta | 18 | |||
Cxcl11 | 93 | F | tgctgagatgaacaggaaggt | 21 | NM_019494.1 |
R | cgcccctgtttgaacataag | 20 | |||
Col1a2 | 69 | F | ctggtgcacagggtgtga | 18 | NM_007743.3 |
R | ctcctgcttgacctggagtt | 20 | |||
Arnt2 | 104 | F | tgcacttcggaaaactccat | 20 | NM_007488.3 |
R | cgagagcccatacacatgc | 19 | |||
Fgf7 | 78 | F | ttactccatagttctgcaaccagt | 24 | NM_008008.4 |
R | tgttgcccttcccttcataa | 20 | |||
Mmp2 | 75 | F | gggcttctgtcctgacca | 18 | NM_008610.3 |
R | aagttcttggtgtaggtgtagatcg | 25 | |||
Bmp8b | 70 | F | ctgtatgaactccaccaaccac | 22 | NM_028189.3 |
R | ggggatgatatctggcttca | 20 | |||
Cdh3 | 75 | F | aggcccagctaacacatgac | 20 | NM_001037809.5 |
R | acaaggccacggtgtctc | 18 | |||
Cdh4 | 60 | F | ttcctggctgctgacaatg | 19 | NM_009867.3 |
R | gtagatctgcagggtcccagt | 21 | |||
Gdf5 | 73 | F | tttattgacaaagggcaagatg | 22 | NM_008109.3 |
R | aggcactgatgtcaaacacg | 20 | |||
Cd80 | 127 | F | ttcgtctttcacaagtgtcttca | 23 | NM_009855.2 |
R | tgccagtagattcggtcttca | 21 | |||
Cldn1 | 92 | F | actccttgctgaatctgaacagt | 23 | NM_016674.4 |
R | ggacacaaagattgcgatcag | 21 | |||
Lefty1 | 92 | F | actcagtatgtggccctgcta | 21 | NM_010094.4 |
R | aacctgcctgccacctct | 18 | |||
Cysltr1 | 95 | F | aaggtgctgaggtaccagatagag | 24 | NM_021476.5 |
R | aatcacagcccttgagaagc | 20 | |||
Drd4 | 137 | F | cccaccaactacttcatcgtg | 21 | NM_007878 |
R | gccatgagcgtgtcacag | 18 | |||
Aldh1a7 | 85 | F | gtttgcagatgccgacttg | 19 | NM_011921.2 |
R | cgctgcaacacaaatctgac | 20 | |||
Cbr2 | 109 | F | gcccatgtcacctttcctaa | 20 | NM_007621.2 |
R | ttacccggatcttgtgtgg | 19 | |||
B3gnt3 | 115 | F | gcaaatacaaccgactgctg | 20 | NM_028189.3 |
R | cactccaggaaaaggacctg | 20 | |||
Cbr3 | 60 | F | aacgttagcgggagagatga | 20 | NM_173047.3 |
R | cccttgatgtgggaaagaatc | 21 | |||
Ldhb | 86 | F | gtagtgggcgttggacaagt | 20 | NM_001316322.1 |
R | acatccaccagggcaagtt | 19 | |||
Gatm | 103 | F | ggtgcactacatcggctctc | 20 | NM_025961.5 |
R | acaggaatttcgggaggaa | 19 | |||
Cacna1d | 60 | F | gaagctgcttgaccaagttgt | 21 | NM_001302637.1 |
R | aacttccccacggttacctc | 20 | |||
Ccl7 | 91 | F | ttctgtgcctgctgctcata | 20 | NM_013654.3 |
R | ttgacatagcagcatgtggat | 21 | |||
MAPK13 | 63 | F | caggctggccttgagtctt | 19 | NM_011950.2 |
R | ccagggctacacagtaagatcc | 22 | |||
Adcy7 | F | gagccttccagacgtccat | 19 | NM_007406.2 | |
70 | R | aggaggataacggcattgg | 19 |