Fig 9. Huh7.5.1–8 cells developed larger numbers of YFV plaques than Vero cells.

Representative black-and-white inverted images of YFV plaques in Huh7.5.1–8 (Huh) and Vero cells. Cells were infected with YFV (17D-204 strain) at either 40 or 16 PFU (determined with Vero cells) per well and then fixed and stained at the indicated times. Values below the images indicate plaque numbers expressed as the mean ± SD of triplicates from one representative experiment. Statistical significance was determined by an unpaired two-tailed t test. Values in parentheses indicate p values, and those less than 0.05 were considered statistically significant. Similar results were obtained in two other independent experiments (S9 Fig). N.D., not determined.