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Panel A shows an example network in the Systems Biology Graphical Notation format (SBGN, www.sbgn.org) [20] to illustrate the basic principle of ScalaFlux. The flux models (and associated datasets) required to quantify the flux through reaction r16 using classical non-stationary 13C-MFA and ScalaFlux are compared in panel B. The ScalaFlux model, the set of measurements required for the flux calculation, and the flux calculation workflow are shown in panel C.
