Fig 5. ¹³C-metabolic flux analysis of prenyl pyrophosphate biosynthesis in Saccharomyces cerevisiae (wild type, S037 and S023 strains).

The yeast prenyl pyrophosphate pathway contains five reactions for the successive condensation of IPP (in grey) onto each intermediate (DMAPP, FPP, GPP and GGPP) (A). The labeling dynamics of IPP were fitted with a double logistic function, which was used as the local label input. Fluxes were estimated by fitting the metabolite concentrations and transient ¹³C-enrichments of GPP, FPP and GGPP. Experimental and fitted data are shown for each strain in panel B for the labeling dynamics (dots: experimental values; lines: best fit) and in panel C for the metabolite concentrations. The fluxes estimated in each strain are given with their standard deviations in panel D. The GGPP demand calculated from phytoene accumulation in strain S023 is shown in grey for comparison. The GGPP turnover rate estimated in each strain is shown in panel E.