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. 2020 Apr 24;6(17):eaaz3154. doi: 10.1126/sciadv.aaz3154

Fig. 2. The NALCN channel complex is selective for small monovalent cations.

Fig. 2

(A) Representative current traces of HEK-293T cells expressing NALCN* + 79 + 80 + 155 under various bi-ionic conditions in response to the voltage protocol shown. EC, extracellular; IC, intracellular. (B) Representative current traces obtained with intracellular NMDG+ and different extracellular mono- or divalent cations using voltage-step protocols shown on the left. (C) The top panel shows currents obtained with a voltage ramp protocol (−80 to 80 mV) with different test ions for both wild-type (WT) [EEKE selectivity filter (SF), top] and the DEKA SF mutant (bottom) using intracellular NMDG+ solution; insets show only −20- to +80-mV range. (D) Reversal potentials of different test ions and their permeability relative to Na+; data are shown as mean ± SD; gray dashed lines indicate 0 nA; numbers in parentheses indicate number of individual cells used for recordings.