Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus) | FVB | Charles River, France | inbred | |
| Strain, strain background (Mus musculus) | CD1 | Charles River, France | outbred | |
| Strain, strain background (Mus musculus) | DUHi | MRC Mary Lyon centre, Oxfordshire, UK | inbred | |
| Strain, strain background (Mus musculus) | B6.129S6-Shh < tm2(cre/ERT2)Cjt>/J | Jackson Laboratory, Maine, USA | Originally in C57Bl6 background. Backcrossed to CD1 | |
| Strain, strain background (Mus musculus) | B6.129S4-Gt(ROSA)26Sortm1LacZSor/J | Jackson Laboratory, Maine, USA | Originally in C57Bl6 background. Backcrossed to CD1 | |
| Commercial assay or kit | TruSeq RNA Sample Prep Kit v2 | Illumina | RS-122–2001 | Non stranded protocol |
| Commercial assay or kit | DISPASE II (NEUTRAL PROTEASE, GRADE II) | ROCHE/SIGMA | 4942078001 | Used at 10 mg/ml in Hepes/KOH 50mM ph7.7 ; NaCl 150 mM. Incubation at 37°C from 45 min to 1h15 depending on stage. |
| Antibody | ANTI-DIGOXIGENIN AP-CONJUGATE, from sheep | ROCHE/SIGMA | 11093274910 | Fab fragments from polyclonal anti-digoxigenin antibodies (from sheep), conjugated to alkaline phosphatase. Used 1/1200ie |
| Commercial assay or kit | BM PURPLE AP SUBSTRATE, PRECIPITATING | ROCHE/SIGMA | 11442074001 | |
| Commercial assay or kit | DIG RNA Labeling Mixture, 10x | ROCHE/SIGMA | 11277073910 |