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. Author manuscript; available in PMC: 2020 Sep 13.
Published in final edited form as: Science. 2020 Mar 13;367(6483):1230–1234. doi: 10.1126/science.aba3526

Fig. 2. Endogenous Orb2 filament can seed further filament formation.

Fig. 2

(A) Time course of monomeric Orb2 aggregation seeded by filaments obtained from adult fly head. The seed (~1 ng) is undetectable in western. Within 6 hours most monomers are aggregated. (B) Negative-stain electron micrographs of seeded oligomers and filaments. (C) Propagation of seeded aggregation through successive rounds. In first round, different seed (1 ng, 2 ng, 5 ng) to monomer (100 ng) ratio was used. In subsequent rounds only 1 ng of seed was used.