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. 2020 Apr 20;13:3291–3301. doi: 10.2147/OTT.S244652

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© 2020 Cheng et al.

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Exosomal lncRNA RAMP2-AS1 regulates VEGFR2 expression by sponging miR-2355-5p. (A) The relative expression of miR-2355-5p in HUVECs was measured by qRT-PCR. (B) The transfection efficiency of miR-2355-5p mimics or inhibitors were measured by qRT-PCR. (C) Luciferase reporter assay validated the interaction between RAMP2-AS1 and miR-2355-5p. (D) Venn diagram shows candidate targets that were predicted by four online databases. (E, F) qRT-PCR and Western blot analyzed the relative mRNA level and protein level of VEGFR2 in HUVECs treated with Exo/SW1353 and si-RAMP2-AS1. (G, H) qRT-PCR and Western blot analyzed the relative mRNA level and protein level of VEGFR2 in HUVECs treated with Exo/SW1353 and miR-2355-5p mimics. (I, J) qRT-PCR and Western blot analyzed the relative mRNA level and protein level of VEGFR2 in HUVECs treated with Exo/SW1353, si-RAMP2-AS1 and miR-2355-5p inhibitors. (K) Luciferase reporter assay validated the interaction between VEGFR2 and miR-2355-5p. *P<0.05.