Table 2.
Primers Used for Real-Time PCR
| Genes | Sequence (5ʹ-3ʹ) |
|---|---|
| Hu-claudin1 | F: GTGCGATATTTCTTCTTGCAGG |
| R: TTCGTACCTGGCATTGACTGG | |
| Hu-claudin3 | F: CTGCTCTGCTGCTCGTGTCC |
| R: TTAGACGTAGTCCTTGCGGTCGTAG | |
| Hu-claudin4 | F: GGCTGCTTTGCTGCAACTGTC |
| R: GAGCCGTGGCACCTTACACG | |
| Hu-claudin7 | F: CATCGTGGCAGGTCTTGCC |
| R: GATGGCAGGGCCAAACTCATAC | |
| Hu-ALDH1 | F: TGTTAGCTGATGCCGACTTG |
| R: TTCTTAGCCCGCTCAACACT | |
| Hu-Vimentin | F: ATCCAAGTTTGCTGACCTCTCTG |
| R: TTCCAGGGACTCATTGGTTCC | |
| Hu-ZEB2 | F: CGCTTGACATCACTGAAGGA |
| R: CTTGCCACACTCTGTGCATT | |
| Hu-EpCAM | F: CGCAGCTCAGGAAGAATGTG |
| R: TGAAGTACACTGGCATTGACGA | |
| Hu-PIK3CG | F: ATGGAGCTGGAGAACTATAAAC |
| R: GGCTGAATGTTTCTCTCCT | |
| Hu-GAPDH | F: GACCCCTTCATTGACCTCAAC |
| R: CTTCTCCATGGTGGTGAAGA |
Abbreviations: CLBC, Claudin-low breast cancer; PIK3CG, PI3K catalytic subunit gamma; PTX, paclitaxel; TNBC, Triple negative breast cancer; BLBC, basal-like breast cancer; PR, progesterone receptor; ERα, estrogen receptor α; HER2, human epidermal growth factor receptor 2; EMT, epithelial-mesenchymal transition; PI3Ks, Phosphatidylinositol 3-kinases; GPCR, G protein coupled receptor.