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. Author manuscript; available in PMC: 2021 May 1.
Published in final edited form as: Neurogastroenterol Motil. 2019 Nov 26;32(5):e13766. doi: 10.1111/nmo.13766

Figure 3: Analysis of enteric neuronal density and neurotransmitter expression in the stomach and duodenum.

Figure 3:

ENS was examined in the distal antrum, pylorus, and proximal duodenum of young (5-week-old) and adult (5-month-old) mice. Neuronal density was decreased in aged mice in the antrum (n=3 per group, p<0.0001) and duodenum (n=3 per group, p<0.0001). These regions also exhibited fewer enteric ganglia (b, n=3 per group, p<0.0001). Number of neuronal cell bodies per ganglion was reduced in the antrum (c, n=3 per group, p<0.01). The proportion of ChAT+ (d) and nNOS+ (e) neurons was not significantly altered with age, except that the antrum in older mice had a significantly smaller proportion of nNOS+ neurons (e, n=3 per group, n<0.001), resulting in a significantly increased ChAT to nNOS ratio (f, n=3 per group, p<0.01.