Bulky and cumbersome |
Smaller, simpler, portable. |
Specialised thermal cyclers required |
Only a heat block is required |
4–8 h until result |
1 h until result |
Requires skilled technicians |
Requires no specific skill |
Requires an additional reverse transcription step |
Can be performed directly on RNA |
Unstable reactions prone to inhibitors requiring purification steps |
Stable and inhibitors tolerated, and thus purification steps not required |
Detects DNA |
Detects DNA and RNA |
Tested on patient samples |
Less tested on patient samples |