Table 2.
Identification of significantly (P < 0.05) differentially expressed plasma membrane proteins in leaves of populations S1 and R1 at 12 h after 2,4-D treatment, as identified by iTRAQ analysis
| Gene ID (RadishDB) | Response | Fold change | Protein match (UniProt accession no.) |
|---|---|---|---|
| RrC129_p7 | Sc < Rc | 8.0 ± 3.3 | Suppressor of BIR1, SOBIR1 (Q9SKB2)* |
| RrC17573_p1 | Sc < Rc | 6.7 ± 1.4 | L-type lectin domain-containing receptor kinase IV.1-like, LecRK-IV.1/LRK1 (O80939) * |
| RrC22_p12 | Sc < Rc | 16 ± 7.7 | Cationic amino acid transporter 1, CAT1 (Q84MA5) |
| RrC3859_p1 | Sc < Rc | 4.1 ± 0.9 | Putative receptor-like kinase At5g39000 (Q9FID8) |
| RrC4_p6 | Sc < Rc | 12 ± 8.2 | ABC transporter G family member 35, ABCG35 (Q7PC86)* |
| RrC4867_p2 | Sc < Rc | 6.5 ± 0.3 | Probable receptor-like kinase At1g51820 (C0LGG3)* |
| RrC538_p2 | Sc < Rc | 4.0 ± 1.7 | ABC transporter G family member 36, ABCG36 (Q9XIE2)* |
| RrC9574_p1 | Sc < Rc | 7.0 ± 2.8 | Plasma membrane-associated cation-binding protein 1, PCAP1 (Q96262)* |
| RrC22_p11 | Sc > Rc | 1.7 ± 0.6 | ADP-ribosylation factor GTPase activating protein AGD12 (Q9FVJ3) |
| RrC27p_9 | Sc > Rc | 3.0 ± 1.0 | ABC transporter B family member 19, ABCB19 (Q9LJX0)* |
| RrC1115_p3 | Rc > Rt | 7.5 ± 4.6 | Glutamate decarboxylase 2-like, GAD2 (Q42472) |
| RrC1913_p1 | Rc > Rt | 12 ± 4.8 | α-Carbonic anhydrase 4, BCA4 (Q94CE4) |
| RrC2092_p3 | Rc > Rt | 12 ± 3.4 | Remorin (O80837) |
| RrC2357_p1 | Rc > Rt | 4.8 ± 1.3 | Ca-transporting ATPase 8-like, ACA8 (Q9LF79) |
| RrC5541_p4 | Rc > Rt | 4.6 ± 0.3 | 60S ribosomal protein L23-a1, RPL23AA (Q8LD46) |
| RrC640_p7 | Rc > Rt | 22 ± 7.1 | Fasciclin-like arabinogalactan protein 8, FLA8 (O22126) |
| RrC9574_p1 | Rc > Rt | 8.1 ± 0.9 | Plasma membrane-associated cation-binding protein 1, PCAP1 (Q96262) |
| RrC1141_p3 | Rc < Rt | 26 ± 5.1 | ABC transporter G family member 40, ABCG40 (Q9M9E1)* |
| RrC1913_p1 | St > Rt | 23 ± 3.4 | α -Carbonic anhydrase 4, BCA4 (Q94CE4) |
| RrC247_p8 | St > Rt | 6.7 ± 0.9 | Uncharacterized protein At3g61260-like (Q9M2D8) |
| RrC12875_p1 | St < Rt | 15 ± 5.8 | Protein PLANT CADMIUM RESISTANCE 2-like, PCR2 (Q9LQU4)* |
Sc, susceptible control; Rc, resistant control; St, susceptible treated; Rt, resistant treated.
*These poteins were selected for MRM-based quantification in all 13 wild radish populations.