Transcriptome profiling analysis of saur41 quadruple mutants and SAUR41 overexpression lines. (A–D) Venn diagrams illustrating differentially expressed genes in wild-type shoot vs. mutant shoot (A), wild-type root vs. mutant root (B), wild-type shoot vs. overexpression shoot (C) and wild-type root vs. overexpression root (D). Shoot samples had far more differentially expressed genes than root samples. (E–H) In 5-day-old seedlings, qRT-PCR validation of differentially expressed genes identified by transcriptome analysis. For each gene, the expression level in Col-0 was set to l.0. (E) In the shoots of saur41/40/71/72, a set of calcium homeostasis/signalling genes are upregulated. (F) A set of ABA signalling-related genes are differentially expressed in the shoos of the quadruple mutants. (G) Overexpression of SAUR41 decreases the transcription of iron homeostasis genes in roots. (H) Overexpression of SAUR41 increases the transcription of ABA biosynthesis/signalling genes in shoots. Each treatment contained three biological replicates. Error bars represent the s.d. **P < 0.01, *P < 0.05, Student’s t-test.