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Overexpression of SAUR41 led to extensive rhizosphere acidification. Seedlings were grown on B5 medium with normal (A) or one-fifth (B) iron concentrations for 7 d and then transferred to B5 plates containing the pH sensor bromocresol purple (0.003 %) for 2 d. Extensive medium acidification was observed for the roots of OE-SAUR41, especially when the seedlings were from the one-fifth iron concentration medium. A pH colorimetric panel is illustrated.
