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Optimization of RT-LAMP reaction for the detection of the PVS under different temperatures. a Agarose gel electrophoresis of the RT-LAMP products. M-100 bp ladder, lanes 1 to 6 (60, 61, 62, 63, 64 and 65 °C temperature, respectively), lane 7-water control and lane 8-healthy control. b Visualization of the RT-LAMP products under UV excitation at 365 nm. The tubes in (b) from left to right containing the RT-LAMP products and SYBR Green I are the same as those in lanes 1 to 8 in (a)
