Fig. 4.
Comparison of the sensitivities of RT-LAMP and RT-PCR assays in the detection of the PVS. a Sensitivity of RT-PCR using a serial dilution of total RNA. M-1 kb ladder, lanes 1 to 8–10−1 to 10−8 dilutions of total RNA, lane 9-healthy control, lane 10-water control. b Sensitivity of RT-LAMP assay detected by agarose gel electrophoresis using a serial dilution of total RNA. The lanes in (b) from left to right containing the RT-LAMP products from the same dilution of total RNA as in lanes 1–8 in (a), lane 9-healthy control, lane 10-water control. c Sensitivity of RT-LAMP assay detected using UV excitation with the addition of SYBER Green I. The tubes in (c) from left to right containing the RT-LAMP products and SYBR Green I are the same as those in lanes 1 to 10 in (b)