Table 1.
Primer | Nucleotide sequence 5′–3′ | Length | Position in gene | Length of product (bp) |
---|---|---|---|---|
Primer set A | ||||
PVS-LF | TTCCACACGACTGGAGCA | 18 | 567–584 | |
PVS-LB | TCGATTATGTGACTAATGGGGCTGC | 25 | 671–695 | |
PVS-F3 | AAGAATCGCGCGGGATTG | 18 | 526–546 | 237 |
PVS-B3 | CTTGTGGGCATTGTGAGCT | 19 | 747–765 | |
PVS-FIP | GCCGATCCGAAGGTAGCCTATGGAAAGTGTGCAGGCTGTA | 40 | 527–566 | 220 |
PVS-BIP | CGCTTCGCCGCATTTGACACCCTACGTATGAGCCCCTCT | 39 | 708–668 | |
Primer set B | ||||
PVS-LF | TCGAGTCCAAGGGCACTG | 18 | 497–514 | |
PVS-LB | AGTCGTGTGGAATTACATGCTAGTC | 25 | 573–597 | |
PVS-F3 | CAAGCGTGAGTAGCTCTGTT | 20 | 437–456 | 219 |
PVS-B3 | CGAAGCGTGCATTCCATTG | 19 | 637–655 | |
PVS-FIP | CCTCAATCCCGCGCGATTCTTCGGGACTGTGGAGTTCCCAA | 41 | 450–490 | 196 |
PVS-BIP | AAGTGTGCAGGCTGTATGCTCCTGCCGATCCGAAGGTAGC | 40 | 606–646 |