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. 2020 Apr 24;10(5):213. doi: 10.1007/s13205-020-02214-4

Table 1.

List of primers used for RT-LAMP assay

Primer Nucleotide sequence 5′–3′ Length Position in gene Length of product (bp)
Primer set A
 PVS-LF TTCCACACGACTGGAGCA 18 567–584
 PVS-LB TCGATTATGTGACTAATGGGGCTGC 25 671–695
 PVS-F3 AAGAATCGCGCGGGATTG 18 526–546 237
 PVS-B3 CTTGTGGGCATTGTGAGCT 19 747–765
 PVS-FIP GCCGATCCGAAGGTAGCCTATGGAAAGTGTGCAGGCTGTA 40 527–566 220
 PVS-BIP CGCTTCGCCGCATTTGACACCCTACGTATGAGCCCCTCT 39 708–668
Primer set B
 PVS-LF TCGAGTCCAAGGGCACTG 18 497–514
 PVS-LB AGTCGTGTGGAATTACATGCTAGTC 25 573–597
 PVS-F3 CAAGCGTGAGTAGCTCTGTT 20 437–456 219
 PVS-B3 CGAAGCGTGCATTCCATTG 19 637–655
 PVS-FIP CCTCAATCCCGCGCGATTCTTCGGGACTGTGGAGTTCCCAA 41 450–490 196
 PVS-BIP AAGTGTGCAGGCTGTATGCTCCTGCCGATCCGAAGGTAGC 40 606–646