Skip to main content
. 2020 Apr 24;40(4):BSR20200353. doi: 10.1042/BSR20200353

Figure 4. NAD+ treatment increased the number of CD11b+ gr-1+ MDSCs in spleen.

Figure 4

(A) Digital images of mice spinal cords’ sections after CD11b and Gr-1 immunofluorescence staining and the quantification of the expression of CD11b and Gr-1. Green fluorescence represented CD11b and red fluorescence represented Gr-1. (B) Splenocytes were stained with CD11b and Gr-1 Ab and then analyzed by flow cytometry. Representative histograms shown. (C) Digital images of mice spinal cords’ sections after Arginase-1 immunofluorescence staining and the quantification of the expression of arginase-1. Green fluorescence represented CD11b and red fluorescence represented Arginase-1. (D) Digital images of mice spleen sections after arginase-1 immunofluorescence staining and the quantification of the expression of arginase-1. (E) Western blot analysis of Arginase-1 expression in spinal cord. (F) Serum expression level of Arginase-1 analyzed by ELISA (n=10 mice/group). Data presented were the mean ± standard deviation. *P<0.05, **P<0.001.